Isolation and Identification of Bacteria in Flower Bee Pollen

Authors

  • Dinko DINKOV Department of Hygiene and Technology of Animal Foodstuffs, Veterinary Legislation and Management, Faculty of Veterinary Medicine, Trakia University, Stara Zagora

DOI:

https://doi.org/10.48048/wjst.2018.2815

Keywords:

Bee pollen, differentiation, microorganisms, species

Abstract

The main food safety requirements include accurately formulated criteria for the presence of specific microorganisms. The inconsistent literature data about the microbial species diversity in bee pollen during its collection, processing, and storage necessitate integral microbiological examinations. The purpose of this research work was to prove the algorithm for isolation and species level differentiation of bacteria from the Enterobacteriaceae, Pseudomonadaceae, Staphylococcaceae, Bacillaceae, and Micrococcaceae families on fresh and dried flower bee pollen from Bulgaria.

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Author Biography

Dinko DINKOV, Department of Hygiene and Technology of Animal Foodstuffs, Veterinary Legislation and Management, Faculty of Veterinary Medicine, Trakia University, Stara Zagora

Assoc. Prof. Dinko Dinkov in Department of Hygiene and Technology of Animal Foodstuffs, Veterinary legislation and management, Faculty of Veterinary Medicine, Trakia University, 6014 Stara Zagora, Bulgaria.

References

Central Cooperative Union. Industry Standard (IS) 2567111-91. Bee Pollen. 1991, p. 1-7.

S Bogdanov. Pollen: Production, Nutrition and Health: A Review. Available at: http://www.bee-hexagon.net/files/file/fileE/Health/PollenBook2Review.pdf, accessed July 2015.

D Stratev, R Balkanska, DD St Mateev and D Dinkov. Processing, storage, labeling and microbiological hazards of organic bee pollen production. Sci. Tech. 2014; 4, 21-7.

Ordinance №9 of 22 June 2005 on the Ministry of Agriculture and Forest, Issued by the Ministry of Agriculture and Forestry, Official Gazette 54/1.07.2005 (BG). Available at: http://econ.bg/l.l_i.129712_at.5.html, accessed July 2016.

MGR Campos, S Bogdanov, LB Almeida-Muradian, T Szczesna, Y Mancebo, C Frigerio and F Ferreira. Pollen composition and standardization of analytical methods. J. Agr. Res. Bee World 2008; 47, 156-63.

T Shevtsova, M Kacaniova, K Garkava, J Brindza and J Petrova. Contamination of Betula verrucosa Ehrh. pollen by microorganisms, mycotoxins and heavy metals. J. Microbiol. Biotech. Food Sci. 2014; 3, 509-13.

Regulation 1441. Commission Regulation (EC) No 1441/2007 of 5 December 2007 amending Regulation (EC) No 2073/2005 on microbiological criteria for foodstuffs. Offic. J. Eur. Union 2007; 322, 12-29.

S Bogdanov. Contaminants of bee products: Review article. Apidologie 2006; 37, 1-18.

D Dominguez-Valhondo, DB Gil, MT Hernandez and D Gonzalez-Gomez. Influence of the commercial processing and floral origin on bioactive and nutritional properties of honeybee-collected pollen. Int. J. Food. Sci. Tech. 2011; 46, 2204-11.

D Dinkov. Differentiation and antibiotic susceptibility of Pantoea agglomerans isolated from flower bee pollen. East. Acad. J. 2016; 1, 99-108.

GFE Koch. Electivnahrboden fur Staphylokokken. Zentr. Bakteriol. Parasitenk. Abt. I Orig. 1942; 149, 122-4.

D Sawhney. The toxicity of potassium tellurite to Staphylococcus aureus in rabbit plasma fibrinogen agar. J. Appl. Bacteriol. 1986; 61, 149-55.

WEJ Sanders and CC Sanders. Enterobacter spp.: Pathogens poised to flourish at the turn of the Century. Clin. Microbiol. Rev. 1997; 10, 220-41.

S Card, M Pearson and GR Clover. Plant pathogens transmitted by pollen. Aust. Plant Path 2007; 36, 455-61.

J Flores, I Gutiérrez and R Espejo. The role of pollen in chalkbrood disease in Apis mellifera: transmission and predisposing conditions. Mycologia 2005; 97, 1171-6.

M Lacey and J West. A Manual for Catching and Identifying Airborne Biological Particles. Available at: http://www.dbbe.fcen.uba.ar/contenido/objetos/aerobiologia.pdf, accessed July 2016.

C Nunes, J Usall, N Teixidó and I Viñas. Biological control of postharvest pear diseases using a bacterium Pantoea agglomerans (CPA-2). Int. J. Food Microbiol. 2001; 70, 53-61.

I Loncaric, H Heigl, E Licek, R Moosbeckhofer, HJ Busse and R Rosengarten. Typing of Pantoea agglomerans isolated from colonies of honey bees (Apis mellifera) and culturability of selected strains from honey. Apidologie 2009; 40, 40-54.

M Mudryk. Plant-isolated Pantoea agglomerans-new look into potential pathogenicity. Mikrobiol. Z. 2012; 74, 53-7.

A Kratz, D Greenberg, Y Barki, E Cohen and M Lifshitz. Pantoea agglomerans as a cause of septic arthritis after palm tree thorn injury: Case report and literature review. Arch. Dis. Child. 2003; 88, 542-4.

KA Bergman, JP Arends and EH Schölvinck. Pantoea agglomerans septicemia in three newborn infants. Pediatr. Infect. Dis. J. 2007, 26, 453-4.

A Joaquin, S Khan, N Russel and N Fayez. Neonatal meningitis and bilateral cerebellar abscesses due to Citrobacter freundii. Pediatr. Neurosurg. 1991; 17, 23-4.

G Steinkamp, B Wiedemann, E Rietschel, A Krahl, J Giehlen, H Barmeier and F Ratjen. Prospective evaluation of emerging bacteria in cystic fibrosis. J. Cyst. Fibros. 2005; 4, 41-8.

G Berg, L Eberl and A Hartmann. The rhizosphere as a reservoir for opportunistic human pathogenic bacteria. Environ. Microbiol. 2005; 7, 1673-85.

HE Müller. Occurrence and pathogenic role of Morganella-Proteus-Providencia group bacteria in human feces. J. Clin. Microbiol. 1986; 23, 404-5.

K Kodama, K Kimura and K Komagata. Two new species of Pseudomonas: P. oryzihabitans isolated from a rice paddy, and P. luteola isolated from clinical specimens. Int. J. Syst. Bacteriol. 1985; 35, 467-74.

J Freney, W Hansen, J Etienne, F Vandenesch and J Fleurette. Postoperative infant septicemia caused by Pseudomonas luteola (CDC Group Ve-l) and Pseudomonas oryzihabitans (CDC Group Ve-2). J. Clin. Microbiol. 1988; 26, 1241-3.

JWA Bending, PJ Mayes, DE Eyers, B Holmes and TTL Chin. Flavimonas oryzihabitans (Pseudomonas oryzihabitans, CDC Group Ve-2): An emerging pathogen in peritonitis related to continuous ambulatory peritoneal dialysis. J. Clin. Microbiol. 1989; 27, 217-8.

ICV Palazzo, PA Azevedo, C Secchi and ACC Pignatari. Staphylococcus hominis stubs. novobiosepticus strains causing nosocomial bloodstream infection in Brazil. J. Antimicrob. Chemoth. 2008; 62, 1222-6.

M Gilliam and BJ Lorenz. Gram-positive cocci from apiarian sources. J. Invertebr. Pathol. 1983; 42, 187-95.

W Levinson. Review of Medical Microbiology and Immunology. 11th ed. McGraw-Hill, New York, 2010, p. 94-9.

M Gilliam, DW Roubik and BJ Lorenz. Microorganisms associated with pollen, honey and brood provisions in the nest of a stingless bee, Melipona fasciata. Apidologie 1990; 21, 89-97.

M Gilliam. Microbiology of pollen and bee bread: The genus Bacillus. Apidoligie 1979; 10, 269-74.

B Ray and A Bhunia. Fundamental Food Microbiology, Opportunistic Bacterial Pathogens, Molds and Mycotoxins, Viruses, Parasites and Fish and Shellfish Toxins. 5th ed. CRC Press Taylor & Francis, 2014, p. 1-390.

ЕЕ Idris, H Bochow, H Ross and R Borriss. Nutzung von Bacillus subtilis als Mittel für den biologischen Pflanzenschutz. VI. Phytohormonartige Wirkung von Kulturfiltraten von pflanzenwachstumsfördernden Bacillus amyloliquefaciens FZB24, FZB42, FZB45 und Bacillus subtilis FZB37. Z. Pflanzenk. Pflanzen. 2004; 111, 583-97.

R Borriss, XH Chen, C Rueckert, J Blom, A Becker, B Baumgarth, B Fan, R Pukall, P Schumann, C Sproer, H Junge, J Vater, A Pühler and HP Klenk. Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: A proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons. Int. J. Syst. Evol. Microbiol. 2011; 61, 1786-801.

LJ Muhamad, H Ito, H Watanabe and N Tamura. Distribution of Microorganisms in Spices and Their Decontamination by Gamma-irradiation. Agr. Biol. Chem. 1986; 50, 347-55.

RF Rogers. Bacillus isolates from refrigerated doughs, wheat flour, and wheat. Cereal. Chem. 1978; 55, 671-4.

European Food Safety Authority (EFSA). Opinion of the Scientific Panel on biological hazards (BIOHAZ) on Bacillus cereus and other Bacillus spp. in foodstuffs. Eur. Food Saf. Authority J. 2005; 3, 175.

P Korner and P Schmid-Hempel. In vivo dynamics of an immune response in the bumble bee Bombus terrestris. J. Invertebr. Pathol. 2004; 87, 59-66.

BM Sadd and P Schmid-Hempel. Principles of ecological immunology. Evol. Appl. 2009; 2, 113-21.

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Published

2017-07-11

How to Cite

DINKOV, D. (2017). Isolation and Identification of Bacteria in Flower Bee Pollen. Walailak Journal of Science and Technology (WJST), 15(3), 225–234. https://doi.org/10.48048/wjst.2018.2815

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Research Article