In Vitro Laticifer Identification in Young Shoot-Derived Callus of Hevea brasiliensis Muell. Arg.



In vitro laticifer in young shoot-derived callus of Hevea brasiliensis Muell. Arg. (RRIM600 cultivar) was examined histologically and histochemically. Calluses were induced on a 2,4-D (1 mgL-l ) supplemented Murashige and Skoog (MS) culture medium containing different strengths of MS-micronutrients (quarter (¼), half (½) and full) and kinetin (0, 1 and 2 mgL-1). The highest percentage of callus induction (52.78 ± 5.01 %) exhibiting hard and compact calluses was achieved using ½-strength MS-micronutrients without kinetin. Friable calluses were obtained on full-strength MS-micronutrients supplemented with 1 mgL-1 kinetin. Histological details indicated that calluses initiated from the sub-epidermal layers. One-month-old calluses presented thick cell wall laticifers which were mainly randomly arranged and located in separately and aggregately. All in vitro laticifers in the callus were non-elongated which were different from those in the stem. Some callus cells exhibited secondary wall thickening which had structural features similar to those of the vessel.


Hevea brasiliensis, laticifers, callus, tracheary element, development

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