Microbial Community Analysis of Aquatic Gliding Bacteria (Cytophaga-Flavobacterium-Bacteroides) by Fluorescence In Situ Hybridization (FISH)

Theerasak ANANTAPONG; Akkharawit KANJANA-OPAS

Authors

Theerasak ANANTAPONG Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90110
Akkharawit KANJANA-OPAS Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90110

Keywords:

FISH, marine gliding bacteria, designed probes, cytophaga-flavobacterium-bacteroides, marine specimens

Abstract

16S rRNA-targeted oligonucleotide probes for the specific detection of the phylum Cytophaga-Flavobacterium-Bacteroides (CFB) were designed and used to monitor the distribution of gliding bacteria in marine specimens from Songkhla lake. The probes were checked for their specificity and then validated using fluorescent in situ hybridization (FISH) with target microorganisms and non-target microorganisms. Tenacibaculum mesphilum, Rapidithrix thailandica, Aureispira marina, Aureispira maritima, Fulvivirga kasyanovii, and Cytophaga sp. were detected with the probe, and hybridization percentage was less than 50 % for all 6 probes tested. Plant material contained between 8.3×10⁹ to 7.4×10¹⁰ cells ml^-1, seagrass contained between 6.4×10¹⁰ to 6.8×10¹⁰ cells ml^-1, biofilms contained between 2.1×10⁹ to 4.6×10¹¹ cells ml^-1, dead fish contained between 1.9×10¹⁰ to 2.6×10¹⁰ cells ml^-1, seaweed contained between 1.3×10¹⁰ to 1.8×10¹⁰ cells ml^-1 and sponge contained 2.9×10¹⁰ cells ml^-1. Members of the Cytophaga-Flavobacterium group were found in some marine specimens, and biofilm samples, except for SK9 and SK11, and usually formed the largest bacterial group in the marine specimens. The medians of the relative abundances and the absolute cell numbers of the alpha-subclass Proteobacteria detected with the probe ALF1b were significantly higher in the marine samples, except for SK7, SK9, and SK11. The results showed the good applicability of the probes TENA, RAP, AUNA, AUMA, FUL, and CYTO for the specific detection and quantification of T. mesphilum, R. thailandica, A. marina, A. maritima, F. kasyanovii, and Cytophaga sp. in complex environments.

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Author Biography

Theerasak ANANTAPONG, Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90110

Department of Agro-industry, Prince of Songkla University

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