Up-Streaming Process for Glucose Oxidase by Thermophilic Penicillium sp. in Shake Flask

Muhammad Mohsin JAVED; Aroosh SHABIR; Sana ZAHOOR; Ikram UL-HAQ

Authors

Muhammad Mohsin JAVED Institute of Industrial Biotechnology, Government College University, Lahore
Aroosh SHABIR Institute of Industrial Biotechnology, Government College University, Lahore
Sana ZAHOOR Institute of Industrial Biotechnology, Government College University, Lahore
Ikram UL-HAQ Institute of Industrial Biotechnology, Government College University, Lahore

Keywords:

Glucose oxidase, thermophilic, Penicillium, submerged fermentation

Abstract

The present study is concerned with the production of glucose oxidase (GOD) from thermophilic Penicillium sp. in 250 mL shake flask. Fourteen different strains of thermophilic Penicillium sp. were isolated from the soil and were screened for glucose oxidase production. IIBP-13 strain gave maximum extra-cellular glucose oxidase production as compared to other isolates. Effect of submerged fermentation in shaking and static conditions, different carbon sources and incubation period on the production of extra-cellular glucose oxidase was studied. Maximum yield (0.325 U/mL) of extra-cellular glucose oxidase was obtained after 72 hrs of incubation at 45 ^oC using submerged fermentation technique in shaking conditions utilizing sucrose as carbon source.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Author Biography

Muhammad Mohsin JAVED, Institute of Industrial Biotechnology, Government College University, Lahore

Assistant Professor

References

CFB Witteveen, P Van de Vondervoort, K Swart and J Visser. Glucose oxidase overproducing and negative mutants of Aspergillus nidulans. Appl. Environ. Microbiol. 1990; 33, 683-6.

J Fiedurek and A Gromada. Screening and mutagenesis of molds for improvement of the simultaneous production of catalase and glucose oxidase. Enzyme Microbial. Technol. 1997; 20, 344-7.

GA EL-Sherbeny, AA Shindia and YMM Sheriff. Optimization of various factors affecting glucose oxidase activity produced by Aspergillus niger. Int. J. of Agric. Biol. 2005; 7, 953-8.

DG Hatzinikolaou and BJ Macris. Factors regulating production of glucose oxidase by Aspergillus niger. Enzyme Microb. Tech. 1995; 17, 530-4.

MI Alvarez, HM Kalisz, HJ Hecht, KD Aumann, D Schomburg and RD Schmid. The design of enzyme sensors based on the enzyme structure. Biosens. Bioelectron. 1995; 10, 735-42.

M Filipiak, K Fludra and E Gosbciminbska. Enzymatic membranes for determination of some disaccharides by means of an oxygen electrode. Biosens. Bioelectron. 1996; 11, 355-64.

M Vodopivec, M Berovic, J Jancar, A Podgornik and A Strancar. Application of convective interaction media disks with immobilised glucose oxidase for online glucose measurements. Anal. Chim. Acta 2000; 407, 105-10.

CE Field, LF Pivarnik, LSM Barnett and AG Rand Jr. Utilization of glucose oxidase for extending the shelf-life of fish. J. Food Sci. 1986; 51, 66-70.

F Hanft and P Koehler. Studies on the effect of glucose oxidase in bread making. J. Sci. Food Agric. 2006; 86, 1699-704.

Z Zhu, C Momeu, M Zakhartsev and U Schwaneberg. Making glucose oxidase fit for biofuel cell applications by directed protein evolution. Biosens. Bioelectron. 2006; 21, 2046-51.

RV Mikhailova, ZhF Shishko, MI Yasenko and AG Lobanok. Effect of culture conditions on extracellular glucose oxidase production by Penicillium adametzii strain BIM-90. Mikol. Fitopatol. 2000; 34, 48-53.

S Ramachandran, P Fontanille, A Pandey and C Larroche. Gluconic acid: properties, applications and microbial production. Food Technol. Biotechnol. 2006; 44, 185-95.

S De Nicolas-Santiago, C Regalado-Gonzalez, B Garcia-Almendarez, FJ Fernandez, A Tellez-Jurado and S Huerta-Ochoa. Physiological, morphological and mannanase production studies on Aspergillus niger uam-gs1 mutants. Electron. J. Biotechnol. 2006; 9, 50-9.

L Jakubikova, V Farkas, N Kolkarova and M Nemcovic. Conidiation of Trichoderma atroviride isolate during submerged cultivation in a laboratory stirred-tank fermenter. Folia Microbiol. 2006; 51, 209-13.

IC Omar and SL Lee. Fungal Isolation and the production of its biomass in a palm oil medium. Pertanika J. Sci. Technol. 1993; 1, 209-24.

H Konemann and LL Van. Toxicokinetics in fish: accumulation and elimination of six chlorobenzenes by guppies. Chemosphere 1979; 9, 3-19.

GW Snedecor and WG Cochrane. Statistical methods. 7th ed. Iowa State University Press, Iowa, 1980.

QN Bhatti and N Saleem. Characterization of glucose oxidase from Penicillium notatum. Food Technol. 2009; 47, 331-5.

MV Sukhacheva, ME Davydova and AI Netrusov. Production of Penicillium funiculosum 433 glucose oxidase and its properties. Appl. Biochem. Microbiol. 2004; 40, 25-9.

EA Nikol'skaia and NA Iasinchuk. Natural variability of Penicillium vitale Pidopl. et Bilai and characteristics of active variants of the fungus that produce glucose oxidase. Mikrobiol Zh. 1977; 39, 601-10.

RV Mikhailova, LA Zhukovskaya and AG Lobanok. Spontaneous variability of glucose oxidase producing fungus Penicillium adametzii LF F-2044. Prikl. Biokhim. Mikrobiol. 2005; 43, 229-33.

D Rando, GW Kohring and F Giffhorn. Production, purification and characterization of glucose oxidase from a newly isolated strain of Penicillium pinophilum. Appl. Microbiol. Biotechnol. 1997; 48, 34-40.

SV Nochure, MF Roberts and AI Demain. True cellulase production by Clostridium thermocellum grown on different carbon sources. Biotech. Lett. 1993; 15, 641-6.

I Haq, MM Javed and TS Khan. An innovative approach for hyperproduction of cellulolytic and hemicellulolytic enzymes by consortium of Aspergillus niger MSK-1 and Trichoderma viride MSK-10. Afr. J. Biotechnol. 2006; 5, 609-14.

PF Stanbury, A Whitaker and SJ Hall. Principles of Fermentation Technology. 2nd ed. Butterworth Heinemann, 1997, p. 93-105.

AR Jafari, MH Sarrafzadeh, I Alemzadeh and M Vosoughi. Effect of stirrer speed and aeration rate on the production of glucose oxidase by Aspergillus niger. J. Biol. Sci. 2007; 7, 270-5.

HM Hamid, K Rehman, MA Zia and M Asghar. Optimization of various parameters for the glucose oxidase from rice polishing using Aspergillus niger. Biotechnol. 2003; 2, 1-7.

BB Sandip, MV Bule, RS Singhal and L Ananthanarayan. Optimization of Aspergillus niger fermentation for the production of glucose oxidase. Food Biopro. Technol. 2008; 2, 344-52.