Use of Promising Bacterial Strains for Controlling Anthracnose on Leaf and Fruit of Mango Caused by Colletotrichum gloeosporioides

Prakong YENJIT; Wanwilai INTANOO; Chiradej CHAMSWARNG; Jingtair SIRIPANICH; Warin INTANA

Authors

Prakong YENJIT Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140
Wanwilai INTANOO Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140
Chiradej CHAMSWARNG Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140
Jingtair SIRIPANICH Department of Horticulture, Faculty of Agriculture at Kamphaengsaen, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140
Warin INTANA School of Agricultural Technology, Walailak University, Thasala, Nakhon Si Thammarat 80161

Keywords:

Antagonist, Colletotrichum gloeosporioidesMango disease, Disease suppression, Biocontrol

Abstract

A total 146 isolates of bacteria were taken from leaf surface, fruit skin, and blossom of mango (var. Nam Dorkmai). They were tested for the inhibition of mycelial growth of Colletotrichum gloeosporioides, a causal agent of anthracnose, on potato dextrose agar (PDA). Seventy-four bacterial isolates inhibited the growth of fungal mycelia by 24.51-49.10%. The 40 highly effective isolates out of 74 isolates were further tested for the potential to reduce the development of anthracnose lesion on detached leaves of mango marcotages at 24 h after inoculation of pathogen. Results indicated that 12 isolates provided high efficacy for inhibiting disease by 51.39-86.11%. Application of these bacteria on mango fruits at 24 h prior to the inoculation of the pathogen revealed that isolates B46 and B12 suppressed disease by 50.36 and 44.13% respectively while Trichoderma harzianum CB-Pin-01 provided 37.30% of the inhibition. For controlling post-harvest disease, an isolate B12 or B12 integrated with hot water treatment (55 oC) provided 91.33 and 88.00% of disease severity reduction respectively when applied at 24 h before inoculation of pathogen. Isolates B12 and B44 were identified as Bacillus subtilis while B46 and K112 were B. licheniformis and B. cereus respectively. The mechanism of these isolates for controlling C. gloeosporioides was the reduction of spore germination and the inhibition of germ-tube elongation.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Sutton BC. The Coelomycetes. Commonweath Mycological Institute, Kew, 1980, 696 p.

Wahid OAA. Occurrence of Colletotrichum gloeosporioides disease of grave fruit in Egypt. International Journal Pest Management. 2001; 47(2): 147-52.

Jeger MJ Plumpley RA Prior C Persad C. SS2-Post-harvest aspects of crop protection. Manila (Philippines) 1987; Agris Accession no. 90-086360.

McDonald FD. Management of post-harvest diseases of tropical fruits and ornamentals in the coribbean region. Walmsley, D. Caribbean Agricultural Research and Development Inst 1992; p. 113-120. Agris Accession no. 2000-057645.

Estrada AB Ilag LL. Effect of temperature and humidity on germination and infection of Colletotrichum gloeosporioides (Penz.) Sacc. on corabao mango. Bacolod city (Philippines) 1990; Agris Accession no. 92-004326.

Dodd JC Estrada AB Matcham J Jeffries P Jeger MJ. The effect of climatic factors on Colletotrichum gloeosporioides causal agent of mango anthracnose in the Philippines. Plant Pathol 1991b; 40: 568-75.

Fitzell RD Peak CM. The epidemiology of anthracnese disease of mango: inoculum sources spore production and dispersal. Annals of Applied Bio 1984; 104: 53-9.

Quimio AJ Quimio JH. Postharvest control of Philippine mango anthracnose by benomyl. Philippine Agriculturist 1974; 58: 147-55.

Nascimento SRC Araujo-Neto SE Hafle OM. Use of prochloraz, azoxystrobin and sodium bicarbonate for the postharvest control of Colletotrichum gloeosporioides Tommy Alkins. Summa Phytopathologica 2000;26(3):379-82.

Dodd JCR Bugante Koomen I Jeffries P Jeger MJ. Pre- and post-harvest control of mango anthracnose in the Philippines. Plant Pathol 1991a; 40: 576-83.

McMillan RT. Control of anthracnose and powdery mildew of mango with systemic and non-systemic fungicides. Trop Agric (Trinidad) 1973; 50: 245-8.

Sampaio VR Demetrio CGB Barbin D. Heat treatment of mango. I. Variation in temperature and time of immersion. Anais-da-Escola-Superior-de-Agricultura- “Luizde-Queiroz”. 1979; 36:659-69. CAB Abstracts. Accession no. 801369738.

Spalding DH Reeder WF. Controlling market diseases of mango with heated benomyl. Proc Florida State Hort Soc 1978; 91: 186-7. CAB Abstracts. Accession no. 790379314.

Chuang TY Ann PI. Biological control of mango anthracnose. Plant Prot Bull Toip 1997; 39: 227-40.

Villiers de EE Korsten L. Alternative strategies for control of mango fruit diseases. Yearbook South African Mango Growers Association. 1996; 16:61-4.

Koomen I Jeffries P. Effects of antagonistic microorganisms on the post-harvest development of Colletotrichum gloeosporioides on mango. Plant Pathol 1993; 42: 230-7.

Korsten L De-Villiers EE Lonsdale JH De-Villiers EE. Biological control of mango postharvest disease in the packhouse. Yearbook-South African Mango Grower’ Association. 1993; 13: 117-21.

Kosten L Lonsdate IH De-Villers EE De-Jager ES. Preharvest biological control of mango diseases. Yearbook-South African Mango Growers Association. 1992; 12:72-8.

Agrios GN. Plant Pathology. Academic Press, New York, 1978.

Gould AB Kobayashi DY Bergen MS. Identification of bacteria for biological control of Botrytis cinerea on petunia using a petal disk assay. Plant Dis 1996; 80: 1029-33.

Schaad NW. Laboratory guide for identification of plant pathogenic bacteria. The Amer Phytopathol Soc, St. Paul, Minnesota 1980, 72 p.

Johnson LF Curl EA. Methods for research on the ecology of soil borne plant pathogen. Burgess Publishing Company, Minnesota, 1972, 247 p.

Schaad NW Jones JB Chun W. Laboratory guide for identification of plant pathogenic bacteria. The Amer Phytopathol Soc, St. Paul, Minnesota 2001, 373 p.

Riker AJ Riker RS. Introduction to research on plant disease. John S Swift Co, St. Louis, Mo. 1936, 117 p.

Fravel DR Spurr HW. Biocontrol of tobacco brown spot disease by Bacillus cereus subsp. Mycoides in a controlled environment. Phytopathology 1977; 67: 930-2.

Yoshida S Hiradate S Tsukamoto T Hatakeda K Shirata A. Antimicrobial activity of culture filtrate of Bacillus amyloliquefacien RC-2 isolated from mulberry leaves. Phytopathology 2001; 91: 181-7.

Baker CJ Stavely JR Thomas CA Sasser M MacFall JS. Inhibitory effect of Bacillus subtilis on Uromyces phaseoli and on development of rust on bean leaves. Phytopathology 1983; 73: 1148-52.

Stessel GJ Leben C Keitt GW. Partial purification and properties of the antifungal antibiotic, toximycin. Phytopathology 1953; 43: 23-6.

Gueldner RC Reilly CC Pusey PL Costello CE. Isolation and identification of iturins as antifungal peptides in biological control of peach brown rot with Bacillus subtilis. J Agric Food Chem 1988; 36: 366-70.

McKeen CD Reilly CC Pusey PL. Production and partial characterization of antifungal substances antagonistic to Monilinia fructicola from Bacillus subtilis. Phytopathology 1986; 76: 136-9.

Asante GS Neal AL. Characterization of fungistatic substance produced by a Bacillus antagonistic to Ceratocystis ulmi. Phytopathology 1963; 54: 819-22.

Fiddaman PJ Rossall S. The production of antifungal volatiles by Bacillus subtilis. J Appl Bacteriol 1993; 74: 119-26.

Pusey PL Wilson CL. Postharvest biological control of stone fruit brown rot by Bacillus subtilis. Plan Dis 1984; 68: 753-6.