Agrobacterium-mediated Transformation of Mungbean [Vigna radiata (L.) Wilczek]

Authors

  • Potjamarn SURANINPONG School of Agricultural Technology, Walailak University, Thasala, Nakhon Si Thammarat 80161
  • Sontichai CHANPRAME Department of Agronomy, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140
  • Hyeon-JE CHO Maxygen Inc3., 515 Galveston Drive, Redwood City, CA 94063
  • Jack M. M WIDHOLM Department of Crop Sciences4, University of Illinois, Urbana, IL 61801
  • Aree WARANYUWAT School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000

Keywords:

Agrobacterium rhizogenes, Agrobacterium tumefaciens, Cholesterol oxidase (choA) gene, Insect control protein, Hairy root, Mungbean transformation

Abstract

Plasmid pCAMBIA 1301-choA was transformed into Agrobacterium rhizogenes strain K599 and A. tumefaciens strain EHA 105 for mungbean transformation. Cotyledons from different ages of mungbean seedlings were inoculated with both bacteria. The two-day-old cotyledons that were co-cultured with hairy root bacteria showed higher ability to produce branched roots than the others. An average of 10 branched roots was formed on both the wounded abaxial site and the hypocotyl cut end. Ten of 75 individual lines (13.25%) showed GUS positive. In addition, cotyledons that were cut and cultured on MB medium supplemented with 2 mgml-1 BAP for 4 days before co-culture with A. tumefaciens using hairy root method revealed high transformation ability (31.25%). It was found that seed age, cultivar, co-culture conditions and culture conditions were important factors affecting the transformation efficiency.

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References

Sarikarin N Srinives P Kaveta R Saksoong P. Effect of seed texture layer on bruchid infestation in mungbean Vigna radiata (L.) Wilczek. ScienceAsia 1999; 25: 203-6.

Purcell JP Greenplate JT Jennings MG Ryerse JJ Pershings JC Sims SR Prinsen MJ Corbin DR Tran M Sammons RD Stonard RJ. Cholesterol oxidase: A potent insecticidal protein active against boll weevill larrae. Biochem Biophys Res Comm 1993; 196: 1406-13.

Corbin DR Greenplate JT Wong EY Purcell JP. Cloning of an insecticidal cholesterol oxidase gene and its expression in bacteria and in plant protoplasts. Appl Environ Microbiol 1994; 60: 4239-44.

Cho HJ Choi KP Yamashia M Morikawa H Murooka Y. Introduction and expression of the Streptomyces cholesterol oxidase gene (ChoA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells. Microbiol Biotechnol 1995; 44: 133-8.

Murashige T Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 1962; 15: 473-97.

Sambrook J Fritsch EF Manniatis T. Molecular cloning : a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, New York, 2000.

Cho HJ Farrand SK Noel GR Widholm JM. High-efficiency induction of soybean hairy roots and propagation of the soybean cyst nematode. Planta 2000; 210: 195-204.

Gamborg O Miller RA Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 1968; 50: 151-8.

Rech FL Golds TJ Husnain T Vainstein MH Jones B Hammatt N Mulligan BJ Davey MR. Expression of a chimaric kanamycin resistance gene introduced into the wild soybean Glycine canescens using a cointegrate Ri plasmid vector. Plant Cell Rep 1989; 8: 33-6.

Fontana GS Santini L Caretto S Frugis G Mariotti D. Genetic transformation in grain legume Cicer arietinum L. (chickpea). Plant Cell Rep 1993; 12: 194-8.

Cho HJ Widholm JM Tanaka N Nakanishi Y Murooka Y. Agrobacterium rhizogenesmediated transformation and regeneration of legume Astragalus sinicus (Chinese milk vetch). Plant Sci 1998; 138: 53-65.

Golds TJ Lee JY Husnain T Ghose TK Davey MR. Agrobacterium rhizogenesmediated transformation of the forage legumes Medicago sativa and Onobrychis vicifolia. J Exp Bot 1991; 42: 1147-57.

Petit A Stougaard J Kuhle A Marcker KA Tempe J. Transformation and regeneration of the legume Lotus corniculatus: a system for molecular studies of symbiotic nitrogen fixation. Mol Gen Genet 1987; 207: 245-50.

Saitou T Kamada H Harada H. Involvement of phytohormones in light-induced adventitious shoot formation of horseradish hairy roots. Plant Sci 1992; 86: 161-6.

Kumar V Jones B Davey MR. Transformation by Agrobacterium rhizogenes and regeneration of transgenic shoots of the wild soybean Glycine argyrea. Plant Cell Rep 1991; 10: 135-8.

Santarem ER Trick HN Essig JS Finer JJ. Sonication-assisted Agrobacterium-mediated transformation of soybean immature cotyledons: optimization of transient expression. Plant Cell Rep 1998; 17: 752-9.

Kudirka DT Colburn SM Hinchee MA Wright MS. Interactions of Agrobacterium tumefaciens with soybean (Glycine max L. Merr.) leaf explants in tissue cultures. Can J Genet Cytol 1986; 28: 808-17.

Fullner KJ Nester EW. Temperature effects the T-DNA transfer machinery of Agrobacterium tumefaciens. J Bacteriol 1996; 178: 1498-504.

Jaiwal PK Kumari R Ignacimuthu S Potrykus I Sautter C. Agrobacterium tumefaciensmediated genetic transformation of mungbean (Vigna radiata L. Wilczek)-a recalcitrant grain legume. Plant Sci 2001; 16: 239-47.

Godwin I Todd G Ford LB Newbury HJ. The effects of acetosyringone and pH on Agrobacterium-mediated transformation vary according to plant species. Plant Cell Rep 1991; 9: 671-5.

Mansur EA Lacorte C De Freitas VG De Oliveira DE Timmerman B Cordeiro AR. Regulation of transformation efficiency of peanut (Arachis hypogaea L.) explants by Agrobacterium tumefaciens. Plant Sci 1993; 89: 93-9.

Cheng M Jarret RL Li Z Xing A Demski JW. Production of fertile transgenic peanut (Arachis hypogaea L.) plants using Agrobacterium tumefaciens. Plant Cell Rep 1996; 15: 653-7.

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Published

2011-12-10

How to Cite

SURANINPONG, P., CHANPRAME, S., CHO, H.-J., WIDHOLM, J. M. M., & WARANYUWAT, A. (2011). Agrobacterium-mediated Transformation of Mungbean [Vigna radiata (L.) Wilczek]. Walailak Journal of Science and Technology (WJST), 1(2), 38–48. Retrieved from https://wjst.wu.ac.th/index.php/wjst/article/view/184

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Research Article