Phosphate Transporter Based Genetic Diversity of Eleusine coracana L., Varieties

Chandra Mohan MEHTA; Kajal SRIVASTAVA; Tapan MOHANTA; Anil Kumar SHARMA

Authors

Chandra Mohan MEHTA Department of Agronomy, School of Agriculture, Lovely Professional University, Punjab
Kajal SRIVASTAVA Department of Biological Sciences, College of Basic Science and Humanities, Uttarakhand
Tapan MOHANTA Department of Biological Sciences, College of Basic Science and Humanities, Uttarakhand
Anil Kumar SHARMA Department of Biological Sciences, College of Basic Science and Humanities, Uttarakhand

Keywords:

Finger millet, EST, phosphate transporter, SSR, PCR, UPGMA, Genetic fingerprint

Abstract

The present study aimed to characterize finger millet (Eleusine coracana L.) varieties on the basis of their phosphate transporter gene which is considered a reliable gene marker for the identification and discrimination in various genotypes. The analysis had 7 different phosphate transporter based SSR markers which were developed and tested against 47 finger millet varieties. Fingerprint analysis showed that germplasm VHC3752 and PRM1 Ragi, PRM 6118 Ragi and PRM 6122 Ragi and, Ragi local Korchara and PRM 5103 were closely located where as VHC 3611 and VHC3972 were very distantly located in the phylogenetic tree. Principle Component Analysis (PCA) of the obtained fingerprint also confirmed genetic diversity among various finger millet varieties. Therefore, this study could be proposed as a reliable tool for characterization of different finger millet varieties in a precise and standard manner.

doi:10.14456/WJST.2016.16

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Author Biography

Chandra Mohan MEHTA, Department of Agronomy, School of Agriculture, Lovely Professional University, Punjab

Assistant Professor,

Agriculture (Agronomy)

References

AM Latha, KV Rao and VD Reddy. Production of transgenic plants resistance to leaf blast disease in finger millet (Eleusine coracana L. Gaertn). Plant Sci. 2005; 169, 657-67.

P Panwar, M Nath, VK Yadav and A Kumar. Comparative evaluation of genetic diversity using RAPD, SSR and cytochrome P450 gene based markers with respect to calcium content in finger millet (Eleusine coracana L.). Indian J. Genet. 2010; 89, 121-33.

P Panwar, AK Jha, PK Pandey, AK Gupta and A Kumar. Functional marker based molecular characterization and cloning of resistance gene analogs encoding NBS-LRR disease resistance protein in finger millet (Eleusine coracana). Mol. Biol. Rep. 2011; 38, 3427-36.

B Venkannababu, T Ramana and TM Radhakrisnan. Chemical composition and protein content in hybrid varieties of finger millet. Indian J. Agric. Sci. 1987; 57, 520-2.

MS Chennaveeraiah and SC Hiremath. Cytogenetics of Minor Millets. In: T Tsuchiya and PK Gupta (eds.). Chromosome Engineering in Plants Genetics Breeding and Evolution. Elsevier, Amsterdam, 1991, p. 613-27.

MM Dida, Srinivasachary, S Ramakrishnan, JL Bennetzen, MD Gale and KM Devos. The genetic map of finger millet, Eleusine coracana. Theor. Appl. Genet. 2007; 114, 321-32.

JGK Williams, AR Kubelik, KJ Livak, AJA Rafalski and SV Tingey. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res.1990; 22, 6531-5.

A Jacobson and M Hedren. Phylogenetic relationships in Alisma Alismataceae based on RAPDs, and sequence data from ITS and trnL. Plant Syst. Evol. 2007; 265, 27-44.

M Soller and JS Beckmann. Genetic polymorphism in varietal identification and genetic improvement. Theor. Appl. Genet. 1983; 67, 25-33.

PR Vos, R Hogers, M Bleeker, M Reijans, T Van De Le, M Hornes, A Frijters, J Pot, J Peleman, M Kuiper and M Zabeau. AFLP: A new technique for fingerprinting. Nucleic Acids Res. 1995; 23, 4407-14.

E Zietkiewicz, A Rafalski and D Labuda. Genome fingerprinting by simple sequence repeat SSR-anchored polymerase chain reaction amplification. Genomics 1994; 202, 176-83.

J Becker and M Heun. Barley microsatellites: Allele variation and mapping. Plant Mol. Biol. 1994; 274, 835-45.

S Chao, W Zhang, J Dubcovsky and M Sorrells. Evaluation of genetic diversity and genome wide linkage is equilibrium among US wheat Triticum aestivum L germplasm representing different market classes. Crop. Sci. 2007; 47, 1018-30.

HA Agrama and MR Tuinstra. Phylogenetic diversity and relationships among sorghum accessions using SSRs and RAPDs. Afr. J. Biotechnol. 2003; 2, 334-40.

Y Qi-lun, F Ping, K Ke-cheng and P Guang-Tang. Genetic diversity based on SSR markers in maize Zea mays L. landraces from Wuling mountain region in China. J. Genet. 2008; 87, 287-91.

E Jewell, A Robinson, D Savage, T Erwin, CG Love, GA Lim, X Li, J Batley, GC Spangenberg and D Edwards. SSR primer and SSR taxonomy tree: Biome SSR discovery. Nucleic Acids Res. 2006; 34, 656-9.

CP Hong, SR Choi and YP Lim. SSR-primer generator: A tool for finding simple sequence repeats and designing SSR-primers. Genom. Info. 2011; 9, 189-93.

SA Barber, JM Walker and EH Vasey. Mechanisms for movement of plant nutrients from soil and fertilizer to plant root. J. Agric. Food Chem. 1963; 11, 204-7.

SE Smith, S Dickson and FA Smith. Nutrient transfer in arbuscular mycorrhizas: How are fungal and plant processes integrated? Aust. J. Plant Physiol. 2001; 28, 683-94.

UI Fluegge. Phosphate translocators in plastids. Annu. Rev. Plant Physiol. Plant Mol. Biol.1999; 50, 27-45.

V Loth-Pereda, E Orsini, PE Courty, F Lota, A Kohler, L Diss, D Blaudez, M Chalot, W Nehls, M Bucher and F Martin. Structure and expression profile of the phosphate transporter gene family in mycorrhizal Populus trichocarpa. Plant Physiol. 2011; 156, 2141-54.

DL Lopez-Arredondo, MA Leyva-Gonzalez, SI Gonzalez-Morales, J Lopez-Bucio and L Herrera-Estrella. Phosphate nutrition: Improving low-phosphate tolerance in crops. Annu. Rev. Plant Biol. 2014; 65, 95-123.

SL Dellaporta, J Wood and JB Hicks. A plant DNA minipreparation: Version II. Plant Mol. Biol. Rep. 1983; 1, 19-21.

S Rozen and HJ Skaletsky. Primer3 (primer3_www.cgi v 0.2) 1998, Available at: http://www.genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi, accessed May 2007.

AJ Robinson, CG Love, J Batley, G Barker and D Edwards. Simple sequence repeat marker loci discovery using SSR primer. Bioinformatics 2004; 20, 1475-6.

F Smith, S Mudge, A Rae and D Glassop. Phosphate transport in plants. Plant Soil 2003; 248, 71-83.