Analysis of Swine (Sus scrofa) DNA from Blowfly (Chrysomya megacephala) Larval Guts after Post-Feeding Periods by PCR Technique

Monthira MONTHATONG; Thummachat THONGCHAILTRIWAT

Authors

Monthira MONTHATONG Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002
Thummachat THONGCHAILTRIWAT Forensic Science Program, Faculty of Science, Khon Kaen University, Khon Kaen 40002

Keywords:

Sus scrofa, cytochrome b, blowfly, forensic entomology

Abstract

This research aimed to investigate the DNA of food sources taken from blowfly (Chrysomya megacephala) larval guts, for application in forensic entomology. The muscle samples of domestic swine (Sus scrofa), decomposed in 2 conditions, in air and drowning, were used as case studies. Adult blowflies were allowed to lay eggs on the meat samples. Then, the third instar larvae were identified as C. megacephala, then transferred to non-meat food and collected every 6 h up to 36 h. Total DNA extraction was performed by the Phenol-Chloroform method, and used as DNA templates in a Polymerase Chain Reaction (PCR) test. The PCR amplified swine partial cytochrome b (cyt b) genes with 203 bp expected size. The results showed that the swine DNA from blowfly larvae grown on decayed tissue under air and drowning conditions could be detected by PCR after larvae post-feeding up to 18 and 36 h, respectively. The sequence analysis revealed that the forward and reverse strands undergo 94 and 98 % identities of the swine cyt b gene sequence retrieved from nucleotide databases. This study suggests that mitochondrial DNA of the food source from insect guts can be detected at limited times after feeding, combined with the condition of the decayed tissues.

doi:10.14456/WJST.2016.12

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Author Biography

Monthira MONTHATONG, Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002

Lecturer, Department of Biology

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