The Effects of Cleistanthoside A Tetraacetate Synthesis on Acute Toxicity and Bone Marrow Micronucleus in ICR Mice

Pranom PUCHADAPIROM; Wanwisa HIMAKHUN; Patoomratana TUCHINDA; Lakana HIMAKOUN; Thidarat KOOMSANG; Prasit SUWANNALERT

Authors

Pranom PUCHADAPIROM Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400
Wanwisa HIMAKHUN Department of Pathology and Forensic Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120
Patoomratana TUCHINDA Department of Chemistry, Faculty of Science, Mahidol University, Bangkok 10400
Lakana HIMAKOUN Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400
Thidarat KOOMSANG Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400
Prasit SUWANNALERT Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400

Keywords:

Cleistanthoside A tetraacetate, toxicity, micronucleus, Phyllanthus spp.

Abstract

Phyllanthus taxodiifolius Beille is used in traditional medicine in tropical and subtropical areas. Although it has been used as a folk medicine for a long time, studies on its safety have been limited. In this study, the bone marrow micronucleus and oral toxicity of Cleistanthoside A tetraacetate, a modified arylnaphthalide lignan of Cleistanthoside A from the P. taxodiifolius Beille, were evaluated. Imprinting control region (ICR) female mice were orally administered Cleistanthoside A tetraacetate at doses of 250, 500 and 1,000 mg/kg body weight (BW). Signs of toxicity, bone marrow micronucleus, clinical blood chemistry, and histopathological findings were determined after treatment. No mortality was observed in any of the groups. A significant increase in numbers of bone marrow micronucleus, marked elevation of alkaline phosphatase (ALP), blood urea nitrogen (BUN), and creatinine (CRE) were observed in the mice administered with Cleistanthoside A tetraacetate 1,000 mg/kg BW. These results also correlated with the histopathological scoring of liver and kidney cells in ICR mice. The mice administered with Cleistanthoside A tetraacetate 1,000 mg/kg BW had high toxicity in the liver and kidneys. Long term or chronic toxicity should be further studied for safety.

doi:10.14456/WJST.2015.68

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