Production and Optimization of Exo and Endocellulases from Thermophilic Fungi Scytalidium thermophilum SKESMBKU02

Sriyamjalla SANTOSHKUMAR; Edla SUJATHA; Pabba SHIVAKRISHNA

Authors

Sriyamjalla SANTOSHKUMAR Department of Microbiology, Kakatiya University, Warangal, Telangana
Edla SUJATHA Department of Microbiology, Kakatiya University, Warangal, Telangana
Pabba SHIVAKRISHNA Department of Microbiology, Kakatiya University, Warangal, Telangana

Keywords:

Scytalidium thermophilum, cellulases, optimization, characterization

Abstract

The study aimed at isolation and screening of thermophilic cellulase producer, optimization and stability studies for maximum cellulase production. Fifteen thermophilic cellulase producing fungi were isolated from thermogenic habitats (vegetable market compost, mushroom compost, horse dung, municipal waste, nests of birds, decomposing litter, soils from furnace area, cattle dung, zoo dump, and industrial waste.) of Telangana. All the fungal isolates were screened for their ability to produce cellulases. Scytalidium thermophilum SKESMBKU02 showed the highest cellulase activity in screening and was selected for further studies. The results showed that S. thermophilum SKESMBKU02 found to have high cellulolytic activity at 45 °C and pH 5.0 - 6.0. Optimization of enzyme production was studied in different carbon and nitrogen sources. The endo and exoglucanase activities were higher in media containing glucose as their carbon source followed by xylose. Yeast extract and peptone were good nitrogen sources for endoglucanase and exoglucanase activity respectively. The organism showed maximum dry weight in (NH₄)₂SO₄ and NH₄Cl. The exo and endocellulases produced by the S. thermophilum SKESMBKU02 were highly stable at pH 8.0 and temperature of 75 °C. The results indicate that the endo and exocellulases produced by the fungi were more stable at high temperature and alkaline pH.

doi:10.14456/WJST.2016.2

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Author Biographies

Sriyamjalla SANTOSHKUMAR, Department of Microbiology, Kakatiya University, Warangal, Telangana

department of Microbiology

kakatiya university

naac 'A' APPROVED UNIVERSITY

Edla SUJATHA, Department of Microbiology, Kakatiya University, Warangal, Telangana

department of Microbiology

kakatiya university

naac 'A' APPROVED UNIVERSITY

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